Design of bioreactors and peripheries (Exercise/Practical): - reactor types and geometry
- materials and surface treatment
- agitation system design
- insertion of stirrer
- sealings
- fittings and valves
- peripherals
- materials
- standardization
- demonstration in laboratory and pilot plant
Sterile operation: - theory of sterilisation processes
- different sterilisation methods
- sterilisation of reactor and probes
- industrial sterile test, automated sterilisation
- introduction of biological material
- autoclaves
- continuous sterilisation of fluids
- deep bed filters, tangential flow filters
- demonstration and practice in pilot plant
Instrumentation and control: - temperature control and heat exchange
- dissolved oxygen control and mass transfer
- aeration and mixing
- used gassing units and gassing strategies
- control of agitation and power input
- pH and reactor volume, foaming, membrane gassing
Bioreactor selection and scale-up: - selection criteria
- scale-up and scale-down
- reactors for mammalian cell culture
Integrated biosystem: - interactions and integration of microorganisms, bioreactor and downstream processing
- Miniplant technologies
Team work with presentation: - Operation mode of selected bioprocesses (e.g. fundamentals of batch, fed-batch and continuous cultivation)
|
