Dr.-Ing. Jürgen Fitschen

2018 - 2023        Research Fellow at the IMS

Boehringer Ingelheim Pharma GmbH & Co. KG
Bioprocess Development Biologicals

Binger Strasse 173

55216 Ingelheim am Rhein

Phone +49 7351 54-177970

Mail Dr. Jürgen Fitschen

Research Expertise

  • Large Scale Bioreactors

  • Characterization of Heterogeneities in Aerated Stirred Tank Reactors

  • Characterization of Mixing Time


Publications

[191132]
Title: Characterization Data for the Establishment of Scale-Up and Process Transfer Strategies between Stainless Steel and Single-Use Bioreactors.
Written by: Bernemann, V.; Fitschen, J.; Leupold, M.; Scheibenbogen, K.-H.; Maly, M.; Hoffmann, M.; Wucherpfennig, T.; Schlüter, M.
in: <em>Fluids</em>. (2024).
Volume: <strong>9</strong>. Number: (5),
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DOI: 10.3390/fluids9050115
URL: https://www.mdpi.com/2311-5521/9/5/115
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Abstract: The reliable transfer of bioprocesses from single-use bioreactors (SUBs) of different scales to conventional stainless steel stirred-tank bioreactors is of steadily growing interest. In this publication, a scale-up study for SUBs with volumes of 200 L and 2000 L and the transfer to an industrial-scale conventional stainless steel stirred-tank bioreactor with a volume of 15,000 L is presented. The scale-up and transfer are based on a comparison of mixing times and the modeling of volumetric mass transfer coefficients kLa, measured in all three reactors in aqueous PBS/Kolliphor solution. The mass transfer coefficients are compared with the widely used correlation of van’t Riet at constant stirrer tip speeds. It can be shown that a van’t Riet correlation enables a robust and reliable prediction of mass transfer coefficients on each scale for a wide range of stirrer tip speeds and aeration rates. The process transfer from single-use bioreactors to conventional stainless steel stirred-tank bioreactors is proven to be uncritical concerning mass transfer performance. This provides higher flexibility with respect to bioreactor equipment considered for specific processes.