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Abstract: The growing demand for bio-pharmaceuticals necessitates improved methods for the characterization of stirred tank reactors (STRs) and their mixing heterogeneities. Traditional Eulerian measurement approaches fall short, culminating in the use of Lagrangian Sensor Particles (LSPs) to map large-scale STRs and track the lifelines of microorganisms such as Chinese Hamster Ovary cells. This study investigates the hydrodynamic characteristics of LSPs, specifically examining the effects that the size and position of the Center of Mass (CoM) have on their flow-following capabilities. Two Lagrangian Particle (LP) designs are evaluated, one with the CoM and a Geometric Center aligned, and another with a shifted CoM. The experimental study is conducted in a rectangular vessel filled with deionized water featuring a stationary circular flow. Off-center LPs exhibit higher velocities, an increased number of floor contacts, and moreover, a less homogeneous particle probability of presence within the vessel compared to LPs with CoM and Geometric Center aligned. Lattice Boltzmann Large Eddy Simulations provide complementary undisturbed fluid velocity data for the calculation of the Stokes number 𝑆𝑡 . Building upon these findings, differences in the Stokes number 𝑆𝑡 between the two LP variants of Δ𝑆𝑡 = 0.01 (25 mm LP) and Δ𝑆𝑡 = 0.13 (40 mm LP) are calculated, highlighting the difference in flow behavior. Furthermore, this study offers a more representative calculation of particle response time approach, as the traditional Stokes number definition does not account for non-homogeneous particles, resulting in an alternative Stokes number (Δ𝑆𝑡alt = 0.84 (25 mm LP) and Δ𝑆𝑡alt = 2.72 (40 mm LP)). This study contributes to the improved characterization of STRs through the use of Lagrangian Sensor Particles. Results highlight the implications the internal mass distribution has on LSP design, offering crucial considerations for researchers in the field.