Publications


    2013:

  • Ardao, I., Zeng, A.-P. (2013) In silico evaluation of a complex multi-enzymatic system using one-pot and modular approaches: Application to the high-yield production of hydrogen from a synthetic metabolic pathway, Chemical Engineering Science 87, 183-193.

  • Cumana, S., Ardao, I., Zeng, A.-P., Smirnova, I. (2013) Glucose-6-phosphate dehydrogenase encapsulated in silica-based hydrogels for operation in a microreactor. Eng. Life Sci. DOI: 10.1002/elsc.201300062.

  • Cumana, S., Simons, J., Liese, A., Hilterhaus, L., Smirnova, I. (2013) Immobilization of glucose-6-phosphate dehydrogenase in silica-based hydrogels: A comparative study, Journal of Molecular Catalysis B: Enzymatic, 85-86, 220-228. doi: 10.1016/j.molcatb.2012.09.014.

  • Elleuche S, Antranikian G (2013) Bacterial group III alcohol dehydrogenases – function, evolution and biotechnological applications. OA Alcohol 1(1):3 (invited review).

  • Elleuche S., Fodor K., Klippel B., von der Heyde, A., Wilmanns, M., Antranikian G (2013) Structural and biochemical characterisation of a NAD+-dependent alcohol dehydrogenase from Oenococcus oeni as a new model for industrial biotechnology applications. Appl Microbiol Biotechnol 97:8963-8975.

  • Elleuche, S., Fodor, K., von der Heyde, A., Klippel, B., Wilmanns, M., Antranikian, G. (2013) Group III-alcohol dehydrogenase from Pectobacterium atrosepticum: insights into enzymatic activity and organization of the metal-ion containing region. Appl Microbiol Biotechnol (in press).

  • Elleuche, S., Klippel, B., von der Heyde, A., Antranikian, G. (2013) Comparative analysis of two members of the metal ion-containing group III-alcohol dehydrogenases from Dickeya zeae. Biotechnol Lett 35:725-733.

  • Fried, D. I., Brieler, F. J., Fröba, M. (2013) Designing inorganic porous materials for enzyme adsorption and applications in biocatalysis. ChemCatChem 5, 931-938.

  • Fried, D. I., Tropp, K., Fröba, M. (2013) On the Way to Cofactor Regeneration in Nanopores: Tailoring Porous Materials for Glucose-6-phosphate Dehydrogenase Immobilization. ChemCatChem 5, 862-884.

  • Mosisch, M., Simons, J. R., Hilterhaus, L., Torda, A. E., (2013). Site-directed protein monomerization by a rational combination of sequence and structure information for glucose 6-phosphate dehydrogenase. ACS Biochemistry (in Vorbereitung).

  • Simons, J. R., Mosisch, M., Torda, A. E., Hilterhaus, L. (2013) Influence of the immobilization site of glucose-6-phosphate dehydrogenase on catalytic activity. Journal of Biotechnology, accepted.

  • Simons, J. R., Mosisch, M., Torda, A. E., Hilterhaus, L. (2013a) Site directed immobilization of glucose-6-phosphate dehydrogenase via thiol-disulfide interchange: Influence on catalytic activity of cysteines introduced at different positions. Journal of Biotechnology 167(1) 1–7.

  • Yesil-Celiktas, O., Cumana, S., Smirnova, I. (2013) Silica-based monoliths for enzyme catalyzed reactions in microfluidic systems with an emphasis on glucose 6-phosphate dehydrogenase and cellulose (in prep.).

    • 2012:

  • Fagaschewski, J., Bohne, S., Kaufhold, D., Müller, J., Hilterhaus, L. (2012) Modular micro reaction engineering for carboligation catalyzed by benzoylformate decarboxylase, Green Processing and Synthesis 1, 4, 337–344.

  • Fagaschewski, J., Bohne, S., Sellin, D., Müller, J., Hilterhaus, L. (2012) Komponenten der Mikrofluidik für das Screening von Biokatalysatoren und Prozessbedingungen, Chemie Ingenieur Technik 84, 8, 1345–1346.

  • Fried, D. I., Tropp, K., Fröba, M. (2012) On the way to cofactor regeneration: Tailoring porous materials for glucose-6-phosphate dehydrogenase immobilization, ChemCatChem, doi: 10.1002/cctc.201200416.

  • Hilterhaus, L., Fagaschewski, J., Bohne, S., Kaufhold, D., Müller, J., Trieu, H. K. (2012) Modulare Mikroverfahrenstechnik für BFD-katalysierte Carboligationen, Chemie Ingenieur Technik 84, 8, 1193.

  • Mosisch, M., Simons, J., Hilterhaus, L., Torda, A. (2012) Monomerization of the dimeric glucose-6-phosphate dehydrogenase: Choice of mutation sites and resulting activity, Biochemistry, submitted.

  • Rieckenberg, F., Götz, K., Hilterhaus, L., Liese, A., Zeng, A.-P. (2012) A lean and efficient production process of the hyperthermophilic archaeon Pyrococcus furiosus and hydrogenase-I with integrated downstream processing in pilot plant scale. Eng. Life Sciences, submitted.

  • Schomburg, K.T., Ardao, I. Göötz, K. Rieckenberg, F., Liese, A., Zeng, A.-P., Rarey, M. (2012) Computational Biotechnology: Prediction of competitive substrate inhibition of enzymes by buffer compounds with protein-ligand docking, Journal of Biotechnology 161 (4), 391–401. doi: 10.1016/j.jbiotec.2012.08.002.

  • Simons, J., Mosisch, M., Torda, A., Hilterhaus, L. (2012) Directed Imobilization of glucose-6-phosphate dehydrogenase by cystein-tags and its reversibility, Journal of Biotechnology, submitted.

    • 2010:

  • Chen, Z., Wilmanns, M., Zeng, A.-P. (2010) Structural synthetic biotechnology: from molecular structure to predictable design of cellular processes for industrial strain development, Trends in Biotechnology, doi: 10.1016/j.tibtech.2010.07.004

  • Wurm, M , Schöpke, B., Lutz, D., Müller, J., Zeng, A.-P. (2010) Microtechnology meets systems biology: The small molecules of metabolome as next big targets, Journal of Biotechnology 149, 33-51.

  • Grote, R., Schöpke, B., Zeng, A.-P., Antranikian, G. (2010) Biotransformationsreaktionen in Mikrosystemtechnik - Visionen für die Industrielle Biotechnik. In: "Nach uns, ohne Öl: Auf dem Weg zu nachhaltiger Produktion", Angrick, M. (Ed.), Metropolis-Verlag, Marburg. Pp.129-141.

Conference contributions


    2013:

  • Dreifke, M. Fried, D.I., Fröba, M. (2013) Immobilization of 6-Phosphogluconate dehydrogenase onto mesoporous silica hosts, 25. Deutsche Zeolith-Tagung, Hamburg, Germany.

  • Fried, D.I., Tropp, K., Fröba, M. (2013) On the way to cofactor regeneration in nanopores: Tailoring porous materials for Glucose-6-phosphate dehydrogenase immobilization. International Mesostructured Materials Symposium (IMMS), Awaji Islands, Hyogo, Japan

  • Uth, M.-F., Crüger, A., Herwig, H. (2013) A new partial slip boundary condition for the Lattice-Boltzmann-method. In: Proceedings of the 11th International Conference on Nanochannels, Microchannels, and Minichannels, June 16-19, Sapporo, Japan.

    • 2012:

  • Ardao, I.; Cumana, S.; Martínez, R.; Smirnova, I.; Zeng, A.-P. (2012) Modeling of a microreactor with glucose 6-phosphate dehydrogenase immobilized in silica-based hydrogels. 6th International Congress on Biocatalysis, Hamburg, Germany.

  • Ardao, I.; Zeng, A.-P. (2012) In silico evaluation of complex multi-enzymatic systems: High-yield production of hydrogen as an example. International Workshop on New and Synthetic Bioproduction Systems, Dezember 6-7, Hamburg, Germany.

  • Bohne, S., Schuler, K., Müller, J. (2012) A Robust Thermal Flow Sensor for Micro Bioreactors”, International Workshop on New and Synthetic Bioproduction Systems, Dezember 6-7, Hamburg, Germany.

  • Götz, K., Malekina, T., Hilterhaus, L., Liese, A. (2012) A Modular System for Biohydrogen Production. Annual meeting of the DECHEMA-VAAM-Section Biotransformations: Catalyzing Bio-Economy - Biocatalysts for Industrial Biotechnology, April 24-25, Frankfurt am Main, Germany.

  • Schomburg, K., Ardao, I., Götz, K., Rieckenberg, F., Liese, A., Zeng, A.-P., Rarey, M. (2012) Computational prediction of enzymatic activity in different buffer solutions. ACS Spring Meeting March 25-29, San Diego, CA, USA.

  • Mosisch, M., Simons, J., Hilterhaus, L., Torda, A. (2012) Modifications to glucose-6-phosphate dehydrogenase for industrial applications: predictions and tests. GCC-2012 - 8th German Conference on Chemoinformatics, November 11-13, Goslar, Germany.

  • Schomburg, K., Ardao, I., Götz, K., Rieckenberg, F., Liese, A., Zeng, A.-P., Rarey, M. (2012) Computational prediction of enzyme activity in different buffer solutions. ProcessNet-Jahrestagung und Jahrestagung der Biotechnologen, September 10-13, Karlsruhe, Germany.

  • Simons, J., Mosisch, M., Torda, A., Hilterhaus, L. (2012) Using Protein Engineering for Enhanced Covalent Immobilization of Glucose-6-phosphate Dehydrogenase. 6th International Congress On Biocatalysis (biocat2012), September 2-6, Hamburg, Germany.

  • Uth, M.-F., Herwig, H. (2012) Flow through nano porous media: Defining a benchmark scenario. In: Proceedings of the ASME 2012 10th International Conference on Nanochannels, Microchannels, and Minichannels, July 8-12, Puerto Rico, USA.

  • Uth, M.-F., Herwig, H., Kolnoochenko, A. Gurikov, P. Menshutina, N. Smirnova, I. (2012) A numerical approach to determine the permeability for nano-porous silica gel. In: Proceedings of the 4th International Conference on Porous Media and its Applications in Science, Engineering and Industry, June 17-22, Postdam, Germany.

  • Hilterhaus, L. (2012) Enzymimmobilisierung und Mikroreaktionstechnik als Werkzeuge auf dem zur Etablierung von Bioprozessen. ERASMUS Hochschuljahr 2011/2012 - Mobilität zu Unterrichtszwecken (STA), May 9, University of Chemical Technology and Metallurgy, Sofia, Bulgaria.

  • Hilterhaus, L. (2012) Coupling immobilization and site-directed mutagenesis to improve biocatalyst performance. ERASMUS Hochschuljahr 2011/2012 - Mobilität zu Unterrichtszwecken (STA), May 8, University of Chemical Technology and Metallurgy, Sofia, Bulgaria.

  • Fried, D.I., Tropp, K., Fröba, M. (2012) Tuning porous materials for large-size protein immobilization. Characterization of porous materials. CPM-6 – The 6th International Workshop on Characterization of Porous Materials: From Angstroms to Millimeters, April 30 - May 2, Delray Beach, Florida, USA.

  • Simons, J., Mosisch, M., Torda, A., Hilterhaus, L. (2012) Combining Molecular Engineering and Covalent Immobilization for a Compartmented Enzymatic Reaction Sequence. Annual meeting of the DECHEMA-VAAM-Section Biotransformations: Catalyzing Bio-Economy - Biocatalysts for Industrial Biotechnology, April 24-25, Frankfurt am Main, Germany.

  • Götz, K., Malekina, T., Hilterhaus, L., Liese, A. (2012) A Modular System for Biohydrogen Production. Annual meeting of the DECHEMA-VAAM-Section Biotransformations: Catalyzing Bio-Economy - Biocatalysts for Industrial Biotechnology, April 24-25, Frankfurt am Main, Germany.

  • Schomburg, K., Ardao, I., Götz, K., Rieckenberg, F., Liese, A., Zeng, A.-P., Rarey, M. (2012) Computational prediction of enzymatic activity in different buffer solutions. ACS Spring Meeting March 25-29, San Diego, CA, USA.

  • Elleuche, S, Klippel, B, Antranikian, G (2012) Characterization of novel bacterial alcohol dehydrogenases capable of oxydizing 1,3-propanediol. VAAM-Jahrestagung March 18-21, Tübingen, Germany, Proceedings p. 134.

  • Fried, D. I., Tropp, K., Fröba, M. (2012) Tuning porous materials for large-size protein immobilization. 24. Deutsche Zeolithtagung, March 7-9, Magdeburg, Germany.

    • 2011:

  • Uth, M.-F., Herwig, H. (2011) A micro-sized generic demonstrator for studying synthetic biological processes. In: Proceedings of the ASME 2011 9th International Conference on Nanochannels, Microchannels, and Minichannels, 2011.

  • Schomburg, K., Ardao, I., Götz, K., Rieckenberg, F., Liese, A., Zeng, A.-P., Rarey, M. (2011) Computational prediction of enzymatic activity in different buffer solutions. 7th Status Seminar on Chemical Biology, December 5-6, Frankfurt, Germany.

  • Bohne, S., Fagaschewski, J., Liese A., Hilterhaus, L., Müller, J. (2011) Kontinuierliche Flüssig-Flüssig-Extraktion in Mikrosystemtechnik. Mikrosystemtechnik Kongress, October 10-12, Darmstadt, Germany.

  • Ardao, I., Zeng, A.-P. (2011) In vitro synthetic biosystem for hydrogen production: modelling, design and optimization of modular processes. ECCE/ECAB, 8th European Congress of Chemical Engineering/1st European Congress of Applied Biotechnology, September 25-29, Berlin, Germany.

  • Cumana, S., Ardao-Palacios, I., Bohne, S., Zeng, A.-P., Müller, J., Smirnova, I. (2011) Immobilization of glucose 6-phosphate dehydrogenase in silica-based hydrogels as the first step in the develop of a systematic pathway for the biological production of hydrogen. ECCE/ECAB, 8th European Congress of Chemical Engineering/1st European Congress of Applied Biotechnology, September 25-29, Berlin, Germany.

  • Fagaschewski, J., Simons, J., Bohne, S., Liese, A., Müller, J., Hilterhaus, L. (2011) “Biotransformations in Microfluidic Systems Applying Dissolved and Immobilized Enzymes”, ECCE/ECAB, 8th European Congress of Chemical Engineering/1st European Congress of Applied Biotechnology, September 25-29, Berlin, Germany.

  • Simons, J., Asano, Y., Hilterhaus, L. (2011) Optimization of a Two Step Reaction Sequence Using Covalent Immobilization Techniques. German Japanese Workshop on Enzyme Technology, September 13-15, Toyama, Japan.

  • Mosisch, M., Torda, A. (2011) Circular Permutations: Detecting Evolutionary related Proteins Pairs On Structure Analysis. ISMB–3DSig, "Structural Bioinformatics and Computational Biophysics” (ISMB satellite meeting), July 15-16 Vienna, Austria.

  • Bohne, S., Fagaschewski, J., Hilterhaus, L., Müller, J. (2011) Continuous Liquid-Liquid Extraction in Microsystems Technology. Lab-on-a-Chip European Congress, June 30 - July 1, Hamburg, Germany.

  • Ardao, I., Zeng, A.-P. (2011) A modular approach to the process design of a novel in vitro synthetic pathway for hydrogen production in microreactors. GVC/DECHEMA Vortrags- und Diskussionstagung: "Bioverfahrenstechnik an Grenzflächen", May 30 - June1, Potsdam, Germany.

  • Fagaschewski, J., Kaufhold, D., Bohne, S., Liese, A., Müller, J., Hilterhaus, L. (2011) Modulare µ-Verfahrenstechnik für enzymatische Reaktionssysteme. GVC/DECHEMA Vortrags- und Diskussionstagung: "Bioverfahrenstechnik an Grenzflächen", May 30 - June1, Potsdam, Germany.

  • Elleuche S, Klippel B, Antranikian G (2011) Identification and characterization of a 1,3-propanediol oxidoreductase from Pectobacterium atrosepticum. VAAM-Jahrestagung 2011, April 3-6, Karlsruhe, Germany, Proceedings p. 160

  • Fried, D. I., Götz, K., Liese, A., Fröba, M. (2011) Covalent immobilization of Glucose-6-phosphate dehydrogenase on a mesoporous silica support. 23. Deutsche Zeolithtagung, March 2-4, Erlangen-Nürnberg, Germany.

    • 2010:

  • Mosisch, M., Simons, J., Hilterhaus, L., Torda, A. (2010) Molecular side-chain optimisation of glucose-6-phosphate dehydrogenase for industrial use. 25th German Conference on Bioinformatics, September 20-22, Braunschweig, Germany.

  • Wurm, M., Müller, J., Zeng, A.-P. (2010) Microfluidics meets Systems Biology: Microfluidic Devices for Metabolic Analysis. 14th International Biotechnology Symposium and Exhibition, September 14-18, Rimini, Italia.

  • Cumana, S., Götz, K., Bohne, S., Liese, A., Müller, J., Smirnova, I., Roth, S. (2010) Mesoporous silica monoliths for enzyme-catalyzed reactions in microfluidic systems. ProcessNet Jahrestagung, September 21-23, Aachen, Germany.

  • Ardao, I., Zeng, A.-P. (2010) Process design and optimization of a novel in vitro synthetic pathway for hydrogen bioproduction by means of mathematical modeling. 5th International Congress on Biocatalysis, August 29 - September 2, Hamburg, Germany.

  • Götz, K., Liese, A. (2010) Hydrogenase I from pyrococcus furiosus in a new biosynthetic pathway. 5th International Congress on Biocatalysis, August 29 - September 2, Hamburg, Germany.

  • Klippel, B., Elleuche, S., Antranikian, A. (2010) Identification of enzymes involved in 1,3-propanediol synthesis from Pelobacter carbinolicus and Pectobacterium atrosepticum. In: 5th International Congress on Biocatalysis, Hamburg, Germany, Book of Abstracts, p. 272.

  • Mosisch, M., Simons, J., Hilterhaus, L., Torda, A. (2010) Protein Modifications for Chemical Applications. 5th International Congress on Biocatalysis, August 29 - September 2, Hamburg, Germany.

  • Simons, J., Mosisch, M., Torda, A., Hilterhaus, L. (2010) Enzyme Immobilization and Surface Interaction of Glucose-6-Phosphate Dehydrogenase. 5th International Congress on Biocatalysis, August 29 - September 2, Hamburg, Germany.

  • Herwig, H., Schmandt, B., Uth, M.-F. (2010) Loss Coefficients in Laminar Flows: Indispensable for the Design of Micro Flow Systems. Proceedings of ASME 2010 3rd Joint US-European Fluids Engineering Summer Meeting and 8th International Conference on Nanochannels, Microchannels, and Minichannels ICNMM2010, August 2-4, Montreal, Canada (ICNMM2010-30166).

  • Götz, K., Liese, A. (2010) Practical Application of hydrogenase I from pyrococcus furiosus. 9th International Hydrogenase Conference, June 27 – July 2, Uppsala, Sweden.

  • Mosisch, M., Simons, J., Hilterhaus, L., Torda, A. (2010) Protein Modications for Chemical Applications . EMBO Conference: Catalytic Mechanisms by Biological Systems: at the Interface between Chemistry and Biology, May 5-7, Hamburg, Germany.

Posters